Journal: Translational Psychiatry
Article Title: Prenatal valproic acid on the basis of gestational diabetes also induces autistic behavior and disrupts myelination and oligodendroglial maturation slightly in offspring
doi: 10.1038/s41398-025-03450-z
Figure Lengend Snippet: A Immunofluorescence staining for ERK (green) a, d, g, j ) and for NG2 (red) displayed OPCs b, e, h, k in the gCC in the control c , VPA f , GDM i , and GDM+VPA l groups at PND 28 ( n = 6 ). DAPI (blue) was used as a nuclear counterstain. The proportion m , the area ratio n , and the intensity ratio o of ERK + OPCs in total OPCs were counted. B Immunofluorescence staining for p-ERK (green) a, d, g, j and for NG2 (red) displayed a OPCs b, e, h, k in the gCC in the control c , VPA f , GDM i , and GDM+VPA l groups at PND 28 ( n = 6 ). DAPI (blue) was used as a nuclear counterstain. The proportion m , the area ratio n , and the intensity ratio o of p-ERK + OPCs in total OPCs were counted. C Expression of ERK and p-ERK in the OLN-93 treated with blank, GABA, GABA+Bicuculline, GABA+ CGP52432 , GABA+VPA (low dose), and GABA+VPA (high dose) was evaluated by Western blotting a ( n = 4 ). β-tubulin expression was served as an internal control. The expression of ERK and p-ERK b, c and the ratio of p-ERK to ERK d were counted. D The ratio of p-ERK to ERK in the OLN-93 pretreated with 10 μM U0126 in the normal culture and the HFHG culture. E The relative optical density (OD) value of CCK-8 test in the OLN-93 treated with GABA, GABA+VPA, and U0126+GABA+VPA ( n = 6 ). F The crystal violet staining of the OLN-93 treated with the methods described in E in the normal culture ( b – d ) and the HFHG culture ( e – g ) ( n = 4 ). The cell density for each group was counted a . G Expression of HDAC1, HDAC3, histone H3, and acetyl-histone H3 in the OLN-93 treated with blank, GABA, GABA+VPA, and GABA+VPA+U0126 was evaluated by Western blotting a ( n = 4 ). LaminB expression was served as an internal control. The expression of HDAC3 b and the ratio of acetyl-histone H3 to histone H3 c were counted. H Expression of DUSP5 in the OLN-93 treated with blank, GABA, and GABA+VPA was evaluated by Western blotting a ( n = 4 ). β-tubulin expression was served as an internal control. The expression of DUSP5 were counted b . I The diagram of intracellular effects of VPA and HFHG in OPCs. *** P < 0.001, * P < 0.05, compared with two groups. # P > 0.05, compared with two groups.
Article Snippet: 10 , Antibody , HDAC3 (D2O1K) Rabbit mAb , Cell Signaling Technology , 85057S , Immunoblotting, (1:1000) , USA.
Techniques: Immunofluorescence, Staining, Control, Expressing, Western Blot, CCK-8 Assay
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